Sample Collection
Edible fillet tissue is taken from fresh, not previously frozen fish. Collection and handling must minimize metal contamination and preserve sample integrity before homogenization.
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ICP-OES
US EPA Method 200.11
Overview of "Determination of Metals in Fish Tissue by Inductively Coupled Plasma–Atomic Emission Spectrometry"
US EPA Method 200.11 defines an ICP-AES/OES procedure for naturally occurring and accumulated toxic metals in edible fish tissue (fillet). The method is intended for fresh tissue and covers metals such as Al, Sb, As, Be, Cd, Cr, Cu, Pb, Ni, Se, Tl and Zn.
End-to-End Analytical Process
Sample Preparation
Fish tissue is homogenized, acid-digested to destroy the organic matrix, then diluted/clarified so metals are in solution for ICP-OES analysis.
Analysis & Maintenance
Prepared digestates are analyzed by ICP-AES/OES using recommended wavelengths, background correction, calibration and QC verification.